Instrumentation 6

Microscopy is the study of objects or samples that are too small to be seen by the naked eye. There are several types of microscopy, each with its own advantages and limitations. Here are the main types of microscopy: 1. Optical microscopy: This is the most common type of microscopy, which uses visible light to illuminate a sample. Optical microscopy can be further divided into several subtypes, such as brightfield, darkfield, phase contrast, fluorescence, and confocal microscopy. Optical microscopy is a technique that uses visible light to observe the sample under a microscope. It consists of several components, including an objective lens, an eyepiece lens, and a light source. The working of optical microscopy involves the following steps. The sample to be viewed is prepared by fixing it onto a glass slide and adding a stain or dye to enhance its contrast. The light source, located beneath the sample, emits light that is directed through the condenser lens to focus the light o

Prokaryotic Cell Vs Eukaryotic Cells



Here's a chart comparing the main differences between prokaryotic and eukaryotic cells:

 

Feature

Prokaryotic Cell

Eukaryotic Cell

Nucleus

No true nucleus

True nucleus, enclosed by a nuclear membrane

DNA

Single, circular chromosome

Multiple linear chromosomes

Organelles

Few, no membrane-bound organelles

Many, membrane-bound organelles

Size

Smaller, typically 1-10 micrometers

Larger, typically 10-100 micrometers

Reproduction

Asexual only

Sexual and asexual

Ribosomes

Smaller

Larger

Cell wall

Present in many, composed of peptidoglycan

Present in some, composed of cellulose or chitin

Movement

Flagella made of flagellin

Flagella or cilia made of microtubules

Examples

Bacteria and archaea

Animals, plants, fungi, and protists





Note that not all prokaryotic cells have a cell wall, and not all eukaryotic cells have flagella or cilia. Additionally, these generalizations have some exceptions, such as bacteria with membrane-bound compartments or eukaryotic cells lacking a true nucleus (such as red blood cells).


Prokaryotes and Eukaryotes are two broad categories of living organisms that differ in their structural and functional characteristics. 

Here is a detailed explanation of the differences between the two:

Prokaryotes:

Prokaryotes are unicellular organisms that lack distinct nucleus and membrane-bound organelles. The genetic material in prokaryotic cells is found in the form of a single circular DNA molecule, which is not enclosed within a nucleus. Prokaryotes are generally smaller in size than eukaryotes and have a simpler structure. They are divided into two main groups: bacteria and archaea.

 

Structural features:

Prokaryotic cells have a cell membrane, which encloses the cytoplasm and other internal structures.

The cytoplasm of prokaryotes contains ribosomes, which are responsible for protein synthesis.

The cell wall of prokaryotes is made up of peptidoglycan, a complex polymer that provides structural support to the cell.

Some prokaryotes have flagella, which are used for movement.


Functional features:

Prokaryotes carry out all metabolic processes within a single cell.

Prokaryotes reproduce through asexual reproduction, which involves the division of a single cell into two identical daughter cells.

Prokaryotes have the ability to exchange genetic material with other cells through a process called horizontal gene transfer.

Prokaryotes are found in a wide range of habitats, including soil, water, and the human body.

Eukaryotes:

Eukaryotes are complex, multicellular organisms that have distinct nucleus and membrane-bound organelles. Eukaryotic cells are larger in size than prokaryotic cells and have a more complex structure. Eukaryotes are divided into four main groups: animals, plants, fungi, and protists.

 

Structural features:

Eukaryotic cells have a distinct nucleus, which houses the genetic material in the form of multiple linear chromosomes.

Eukaryotic cells contain a variety of membrane-bound organelles, such as mitochondria, endoplasmic reticulum, and Golgi apparatus, which carry out specialized functions within the cell.

The cytoplasm of eukaryotic cells contains ribosomes, which are responsible for protein synthesis.

Eukaryotic cells are surrounded by a plasma membrane, which separates the internal environment of the cell from the external environment.


Functional features:

Eukaryotes carry out metabolic processes through a variety of specialized cells, tissues, and organs.

Eukaryotes reproduce through both asexual and sexual reproduction, depending on the species.

Eukaryotes have a complex system of growth, development, and differentiation, which allows them to carry out a wide range of specialized functions.

Eukaryotes are found in a wide range of habitats, including soil, water, and the human body.


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