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Showing posts with the label Marine World

Instrumentation 6

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Microscopy is the study of objects or samples that are too small to be seen by the naked eye. There are several types of microscopy, each with its own advantages and limitations. Here are the main types of microscopy: 1. Optical microscopy: This is the most common type of microscopy, which uses visible light to illuminate a sample. Optical microscopy can be further divided into several subtypes, such as brightfield, darkfield, phase contrast, fluorescence, and confocal microscopy. Optical microscopy is a technique that uses visible light to observe the sample under a microscope. It consists of several components, including an objective lens, an eyepiece lens, and a light source. The working of optical microscopy involves the following steps. The sample to be viewed is prepared by fixing it onto a glass slide and adding a stain or dye to enhance its contrast. The light source, located beneath the sample, emits light that is directed through the condenser lens to focus the light o...
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STD 12th / Ch-14/ Explain Zooplankton, Phytoplankton, Benthos.

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  Zooplankton: Zooplankton are small, drifting aquatic animals that live in the water column of oceans , lakes , and rivers . They are a diverse group of organisms that includes many different types of animals, such as tiny crustaceans, jellyfish, and larval forms of larger animals. Zooplankton are important components of aquatic ecosystems , serving as food for larger predators and playing critical roles in nutrient cycling and the carbon cycle . They also have significant impacts on the marine food web, as changes in zooplankton populations can affect the abundance and distribution of higher trophic levels, including fish and marine mammals. Zooplankton can be classified based on their size , with the smallest forms referred to as microzooplankton and the larger forms as microzooplankton . They can also be classified based on their feeding behavior , with some species feeding on phytoplankton and others consuming other zooplankton. Overall, zooplankton are a vital compo...
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Isolation and development of a pure culture of Chlorella sp. and protein estimation.

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[ Note:  experimental data] The most dominant green microalgae, Chlorella sp.,  The axenic unialgal culture was developed by the standardized Streak Plate Method . After that, unialgal cultures growth was optimized in the laboratory in BG11 media (procedure available in the previous post)then high cell density seed cultures were developed for the experimental study ( Fig. 1 ). Figure 1  Schematic representation of isolation and development of an axenic culture of  Chlorella   sp. Growth and biomass production of Chlorella sp. In the present study, different nitrate sources (in the form of potassium nitrate and sodium nitrates) were used to enhance the growth and biomass of Chlorella sp. During 24 days cultivation period . The culture density of  Chlorella    Sp was determined at 680 nm (ʎ max ), and the results were presented in Fig. 2a. During the cultivation period, the density of the culture was linearly increased from 0.005 to 0.86 at 680 nm....
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Impact studies on the growth of a Chlorella Sp. culture using NaNO3/KNO3 as nitrogen sources.

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  The green algae Chlorella sp pure cultures were developed and optimized in modified BG11 media for 24 days under laboratory conditions such as constant temperature (25±1˚C) and light (1000 µmol s -1 m -2 ) 12hr light and 12hr dark periods. After that, axenic seed cultures (2 ml) were inoculated in two triplicate sets of 250 ml flasks containing 100 ml of modified media ( Table 1&2 ) . Then the experimental flasks were kept in laboratory conditions. During the cultivation culture, cell density (growth), biomass, and nutrient consumption were determined over the cultivation time. Media formulation BG11 is a well-known media for green microalgae cultivation. In this study, BG11 media composition was modified to grow Chlorella sp. The preparation of culture media (Tables 1&2) and the salinity (up to 10ppt). The media was divided into two parts, and each part was supplemented with two nitrate sources in the form of sodium nitrate (NaNO 3 ) (20 mM) and potassium nitrate (...
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Microalgae[Chlorella] role in the nitrogen cycle.

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  Microalgae are found in freshwater. Algae require an abundant amount of sunlight in the open air to grow and reproduce. All essential nutrient-containing media support microalgae growth. Microalgae consume and utilize nutrients in cellular metabolism. Chlorella is a well-known freshwater unicellular and free-living microalga, cells are 2 to10 µm in size. The cell wall of Chlorella contains membrane protein. These proteins are involved in different activities like preserving membrane potential and these activity control by Na + and K + -ATPase pumps. Na + and K + -ATPase belongs to the P-type ATPase family. Osmoregulation, nutrient transport, Na + concentration, and regulation of cell volume all materialize in the presence of the Na + and K + -ATPase pumps. Around 30% energy is required for the sodium-potassium movement in between cell membranes. Figure 1. Briefly explain the ion-pumping enzymes that contribute to the fluid balance and its electrolyte ma...
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Structure of Antibody Explain in brief.

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Antibodies , also known as immunoglobulins (Ig), are Y-shaped proteins that are produced by B cells in response to the presence of a foreign substance, known as an antigen . Each antibody molecule is composed of four polypeptide chains, two identical heavy chains, and two identical light chains, which are linked together by disulfide bonds. The basic structure of an antibody molecule consists of two identical antigen-binding fragments (Fab) and a single crystallizable fragment (Fc). The Fab region is located at the tips of the Y-shaped molecule and is responsible for binding to specific antigens. The Fc region is located at the base of the Y and is responsible for the effector functions of the antibody, such as activating complement and binding to Fc receptors on immune cells. Each Fab region contains a variable region and a constant region. The variable region is responsible for binding to the antigen and is highly variable between different antibodies, while the constant region ...
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Impact studies on the growth of a Chlorella Sp. culture using NaNO3/KNO3 as nitrogen sources.

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  The green algae Chlorella sp pure cultures were developed and optimized in modified BG11 media for 24 days under laboratory conditions such as constant temperature (25±1˚C) and light (1000 µmol s -1 m -2 ) 12hr light and 12hr dark periods. After that, axenic seed cultures (2 ml) were inoculated in two triplicate sets of 250 ml flasks containing 100 ml of modified media ( Table 1&2 ) . Then the experimental flasks were kept in laboratory conditions. During the cultivation culture, cell density (growth), biomass, and nutrient consumption were determined over the cultivation time. Media formulation BG11 is a well-known media for green microalgae cultivation. In this study, BG11 media composition was modified to grow Chlorella sp. The preparation of culture media (Tables 1&2) and the salinity (up to 10ppt). The media was divided into two parts, and each part was supplemented with two nitrate sources in the form of sodium nitrate (NaNO 3 ) (20 mM) and potassium nitrate (...
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STD 12th/Ch-5/ Mention the advantage of selecting pea plant for the experiment by Mendel.

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Gregor Mendel selected pea plants for his experiments because they have several advantages that made them an ideal organism for genetic research. Some of the advantages are: Easy to grow and maintain:  Pea plants are easy to grow and can be cultivated in a small area. They have a short life cycle, and many generations can be produced in a short time. A large number of offspring: Pea plants produce a large number of offspring, which allows for statistical analysis and a more accurate determination of genetic ratios. Self-fertilizing: Pea plants can self-fertilize, which means that they can produce offspring by fertilizing their own flowers. This characteristic makes it easier to control and manipulate genetic traits. Clear-cut traits: Pea plants have several easily identifiable traits that are controlled by single genes, such as seed color, flower color, and plant height. These traits are distinct and show clear-cut differences between the two parental varieties. No environmental...
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