Instrumentation 6

Microscopy is the study of objects or samples that are too small to be seen by the naked eye. There are several types of microscopy, each with its own advantages and limitations. Here are the main types of microscopy: 1. Optical microscopy: This is the most common type of microscopy, which uses visible light to illuminate a sample. Optical microscopy can be further divided into several subtypes, such as brightfield, darkfield, phase contrast, fluorescence, and confocal microscopy. Optical microscopy is a technique that uses visible light to observe the sample under a microscope. It consists of several components, including an objective lens, an eyepiece lens, and a light source. The working of optical microscopy involves the following steps. The sample to be viewed is prepared by fixing it onto a glass slide and adding a stain or dye to enhance its contrast. The light source, located beneath the sample, emits light that is directed through the condenser lens to focus the light o

Gregor Mendel

Gregor Mendel was an Austrian monk who is widely regarded as the founder of modern genetics. Mendel conducted a series of experiments on pea plants between 1856 and 1863 to study the inheritance of traits from one generation to the next. These experiments involved crossing different varieties of pea plants and analyzing the patterns of inheritance that emerged.


Mendel's most famous experiment involved crossing pea plants with different traits for flower color (purple or white) and observing the inheritance of these traits in the offspring. He performed a series of crosses between plants with different flower colors and found that the inheritance of the trait followed a predictable pattern, which he later referred to as the laws of inheritance.


Mendel's experiments involved the following steps:

  1. He chose pea plants with clearly defined traits (such as flower color) that could be easily distinguished from one another.
  2. He crossed plants with different traits and observed the characteristics of the offspring.
  3. He analyzed the patterns of inheritance in the offspring and recorded his observations.


Mendel's crosses involved several generations of pea plants, and he carefully tracked the inheritance of the traits in each generation. He observed that certain traits (such as flower color) appeared in some generations but not in others, and he noted the ratios of the different traits in the offspring of each cross.


Mendel's crosses demonstrated that traits are inherited in a predictable manner, with certain traits being dominant over others. He also discovered that traits can be passed down from one generation to the next in the form of discrete units of heredity, which he referred to as "factors" (later known as "genes").


Mendel's work laid the foundation for the field of genetics, and his experiments on pea plants remain a classic example of how the principles of inheritance can be studied and understood.





 

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