Instrumentation 6

Microscopy is the study of objects or samples that are too small to be seen by the naked eye. There are several types of microscopy, each with its own advantages and limitations. Here are the main types of microscopy: 1. Optical microscopy: This is the most common type of microscopy, which uses visible light to illuminate a sample. Optical microscopy can be further divided into several subtypes, such as brightfield, darkfield, phase contrast, fluorescence, and confocal microscopy. Optical microscopy is a technique that uses visible light to observe the sample under a microscope. It consists of several components, including an objective lens, an eyepiece lens, and a light source. The working of optical microscopy involves the following steps. The sample to be viewed is prepared by fixing it onto a glass slide and adding a stain or dye to enhance its contrast. The light source, located beneath the sample, emits light that is directed through the condenser lens to focus the light o

Measures of central tendency and dispersion.

Measures of central tendency are used to describe the central or typical value of a set of data. The three main measures of central tendency are:

 

Mean:

The mean is the average value of a set of data. It is calculated by adding up all of the values in the data set and dividing by the number of observations.

In statistics, the mean is a measure of central tendency that is used to describe the average value of a set of data. It is sometimes referred to as the arithmetic mean, as it is calculated by adding up all of the values in the data set and dividing by the number of observations.

 

The formula for the mean is:


Where,

x1, x2, ..., xn are the individual observations in the data set, and n is the total number of observations.

 

The mean is a useful statistic because it provides a single, representative value that can be used to summarize the data set. It is often used in inferential statistics to make predictions or estimates about a larger population based on a sample of data.

However, the mean can be sensitive to outliers or extreme values in the data set. In some cases, a single extreme value can significantly affect the mean and make it an unreliable measure of central tendency. In such cases, alternative measures such as the median or mode may be more appropriate.

It is also important to note that the mean is only applicable for data that is continuous or discrete, and that can be measured on an interval or ratio scale. It is not applicable for categorical or nominal data, which require different types of measures of central tendency.


 

Median:

The median is the middle value in a set of data when the observations are arranged in order from smallest to largest. If there is an even number of observations, the median is the average of the two middle values.

In statistics, the median is a measure of central tendency that represents the middle value of a dataset when it is arranged in order from lowest to highest or highest to lowest. The median is a robust statistic, meaning that it is not affected by extreme values or outliers in the dataset. It is commonly used in a variety of applications, including economics, finance, biology, and social sciences.

 

The formula for the median is:

 


  

Where,

l= lower limit of the class in which median lies

f= frequency of the class in which median lies

F= cumulative frequency of the class preceding the median class

C= width of the interval in which median lies

Note that here median class is the class-interval in which ()th observation lies.

 

Arrange the data in order from lowest to highest or highest to lowest.

If the dataset contains an odd number of values, the median is the middle value. For example, in the dataset {2, 4, 6, 8, 10}, the median is 6.

If the dataset contains an even number of values, the median is the average of the two middle values. For example, in the dataset {2, 4, 6, 8}, the median is (4 + 6) / 2 = 5.

In some cases, the dataset may contain repeated values that result in the median being a value that is not actually in the dataset. For example, in the dataset {1, 2, 2, 3, 4}, the median is 2, even though there is no value of 2 that occurs exactly in the middle.

One advantage of using the median as a measure of central tendency is that it is less sensitive to outliers than other measures such as the mean. For example, if a dataset contains a few extremely high or low values, the mean may be significantly affected, leading to an inaccurate representation of the data. In contrast, the median is not influenced by these outliers and provides a more robust estimate of the typical value.

The median is also useful when dealing with skewed datasets, which are datasets that are not symmetrical and have more values on one side than the other. In such cases, the median is often a better representation of the center of the data than the mean.

 

 

Mode:

The mode is the most common value in a set of data.

In statistics, the mode is a measure of central tendency that represents the most frequent value in a dataset. It is the value that occurs most often, or the value that has the highest frequency. The mode is useful in describing the central tendency of a dataset, especially when there are many repeated values or a high degree of clustering.

The mode can be calculated for both categorical and numerical data. For categorical data, such as colors or types of animals, the mode is simply the category with the highest frequency. For numerical data, such as test scores or heights, the mode is the number with the highest frequency.

Calculating the mode is a simple process. The data is first arranged in ascending or descending order, and then the value(s) that appear most frequently are identified. If there is only one mode, the dataset is said to be unimodal. If there are two modes, the dataset is bimodal, and if there are more than two modes, the dataset is multimodal.

In some cases, a dataset may not have a mode, or may have several values with the same frequency, resulting in no clear mode. This can happen when the data is evenly distributed or when there are no repeated values.

The mode is useful in a variety of applications, including psychology, sociology, economics, and biology. In psychology, the mode can be used to describe the most common behavior or personality trait. In sociology, the mode can be used to describe the most common demographic characteristic of a group. In economics, the mode can be used to describe the most common price or income level. In biology, the mode can be used to describe the most common physical or genetic trait.

One limitation of the mode as a measure of central tendency is that it can be affected by outliers or extreme values. Unlike the median, which is unaffected by extreme values, the mode can be skewed by these values, leading to an inaccurate representation of the data. Additionally, the mode may not be useful in describing the spread or variability of the data, as it only represents a single value.

 

 

Mode

 



 

l = lower limit of the modal class

f0 = frequency of the class preceding the model class

f1 = frequency of the modal class

f2 = frequency of the class succeeding the modal class

c = width of the modal class

 

Note that modal class means the class – interval having maximum frequency.

 

Measures of dispersion, also known as measures of variability, are used to describe the spread or variability of a set of data. The three main measures of dispersion are the range, variance, and standard deviation.

 

Range:


The range is the simplest measure of dispersion and is defined as the difference between the highest and lowest values in a dataset. It is a quick way to get a sense of how spread out the data is, but it does not take into account the distribution of the data in between the highest and lowest values. For example, if the range of a dataset is 10, that means the difference between the highest and lowest values in the dataset is 10.

 

Variance:

The variance is a more precise measure of dispersion that takes into account the distribution of the data. It is calculated by finding the average of the squared differences between each value in the dataset and the mean. The variance is represented by the symbol σ² (sigma squared) and is expressed in the units of the original data squared. A high variance indicates that the data is spread out over a wider range, while a low variance indicates that the data is more tightly clustered around the mean.

 

Standard Deviation:

 

The standard deviation is the most used measure of dispersion and is the square root of the variance. It is represented by the symbol σ (sigma) and is expressed in the same units as the original data. Like the variance, the standard deviation provides a measure of how spread out the data is, with a high standard deviation indicating that the data is more spread out and a low standard deviation indicating that the data is more tightly clustered around the mean.

 

The choice of which measure of dispersion to use depends on the nature of the data and the purpose of the analysis. The range is useful when a quick estimate of the spread is needed, while the variance and standard deviation are more appropriate for situations where a more precise measure of dispersion is required.

 

 

The mean, median, and mode are all measures of central tendency that are commonly used in statistics. While they are related to each other, they provide different information about the distribution of the data.

 

The mean, also known as the arithmetic mean, is calculated by summing all the values in a dataset and dividing by the number of values. The mean is sensitive to outliers, meaning that extreme values can have a disproportionate impact on the calculation of the mean. When a dataset is approximately normally distributed, the mean provides a good estimate of the central tendency of the data.

The median is the middle value in a dataset when the data is arranged in order from smallest to largest. The median is not sensitive to outliers and provides a more robust measure of central tendency than the mean. The median is useful when the dataset contains extreme values or is not normally distributed.

The mode is the value that occurs most frequently in a dataset. The mode is not sensitive to outliers and provides information about the most common value in the data. The mode is useful for categorical data or when there is a clear peak or cluster in the data.

The relationship between the mean, median, and mode can provide information about the shape of the distribution of the data. When a distribution is symmetrical, the mean, median, and mode are all equal. This is the case for a normal distribution, where the mean, median, and mode are all located at the center of the distribution.

When a distribution is skewed, the mean, median, and mode will be different. In a positively skewed distribution, the mean will be greater than the median and the mode, while in a negatively skewed distribution, the mean will be less than the median and the mode. The direction of the skewness determines whether the mean is greater or less than the median and mode.

 

 I wish all information are helpful to you.

Thank you so much…

Have a Great Day!!!! 


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